Production of WGHs and AFPHs using Protease Combinations at High and Ambient Pressure
نویسندگان
چکیده
Wheat gluten hydrolyzates (WGHs) and anchovy fine powder hydrolyzates (AFPHs) were produced at 300 MPa using combinations of Flavourzyme 500MG (F), Alcalase 2.4L (A), Marugoto E (M) and Protamex (P), and then were compared to those produced at ambient pressure concerning the contents of soluble solid (SS), soluble nitrogen and electrophoretic profiles. The contents of SS in the WGHs and AFPHs increased up to 87.2% according to the increase in enzyme number both at high and ambient pressure. Based on SS content, the optimum enzyme combinations for one-, two-, threeand four-enzyme hydrolysis were determined as F, FA, FAM and FAMP, respectively. Similar trends were found for the contents of total soluble nitrogen (TSN) and TCA-soluble nitrogen (TCASN). The contents of SS, TSN and TCASN in the hydrolyzates together with electrophoretic mobility maps indicates that the high-pressure treatment of this study accelerated protein hydrolysis compared to ambient-pressure treatment. Keywords—Production, Wheat gluten hydrolyzates, Anchovy fine powder hydrolyzates, Protease combinations.
منابع مشابه
The effect of smart water and silica nanoparticles injection on wettability of limestone
Today, by using half of the oil reserves of the world, natural production of oil has decreased drastically. Gas and water injection for maintaining reservoir pressure is not responsive for oil production.At first, we dispersed silica nanoparticles in low salinity and after two weeks, there was no change in its stability. Then we mixed the nanoparticles in low salinity. After a while, we found o...
متن کاملThe effect of smart water and silica nanoparticles injection on wettability of limestone
Today, by using half of the oil reserves of the world, natural production of oil has decreased drastically. Gas and water injection for maintaining reservoir pressure is not responsive for oil production.At first, we dispersed silica nanoparticles in low salinity and after two weeks, there was no change in its stability. Then we mixed the nanoparticles in low salinity. After a while, we found o...
متن کاملA novel low molecular weight extracellular protease from a moderately halophilic bacterium Salinivibrio sp. strain MS-7: production and biochemical properties
Kinetics of bacterial growth and protease production were monitored on a novel isolated moderately halophilic bacterium, Salinivibrio sp. strain MS-7, and maximum growth and protease activity was achieved after 48 hours at 30°C and 180 rpm. To determine the effect of various carbon sources on protease production, glucose, lactose, sucrose and maltose were investigated and maximum production of...
متن کاملProduction of Extracellular Protease and Determination of Optimal Condition by Bacillus Licheniformis BBRC 100053 (RESEARCH NOTE)
The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...
متن کاملEffect of Ambient Condition on the Shower Cooling Tower in Four Type of Climates Condition
Water cooling by ambient takes place with two mechanisms of heat and mass transfer. Using packings at wet cooling towers has disadvantages such as obstruction, reduction of life expectancy and production of algae and fungi. In shower cooling towers types of towers packings are completely removed and water intake is in direct contact and heat transfer takes place in two ways of latent and sensib...
متن کامل